Enter the sequence

# Example for substitution:
CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC
# Example for insertion:
CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC
# Example for deletion:
CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC
#The three examples above represent the input formats of three different editing types.The bases to be edited for each of the three editing types are all marked with a pair of parenthese().
#For base substitution,it is indicated by backslash "/",and the example is used to replace A with T.
#For base insertions are indicated by the plus sign "+",and the example indicates that ATT will be inserted at the parenthesis position
#Base deletions are indicated with a plus sign -. Examples indicate that GCTGGCGCGA will be deleted at the parenthesis position;

 

PAM   Maximum edit-to-nick distance     

Maximum PBS length   Maximum RTT length  

Minimum PBS length   Minimum RTT length  

NUMBER of optimal pegRNAS  Assembly  

MINIMUM NICK-TO-NICK DISTANCE  MAXIMUM NICK-TO-NICK DISTANCE 

MINIMUM DOWNSTREAM HOMOLOGY 

 

History

Download
Strand Spacer PAM PBS RTT EditToNickDistance sgRNASpacer sgRNAPAM NickToNickDistance Efficiency(%) Time
- CCTCGGACAGTAATCTGTCT CGG CAGATTACTGTCCGAGG TTGGGAAATCTGAGA 5 ATCAGACCCAGGGCAATCGG TGG 99 3.7743747234344482 20230217174641
- CCTCGGACAGTAATCTGTCT CGG CAGATTACTGTCCGAGG CTTGGGAAATCTGAGA 5 ATCAGACCCAGGGCAATCGG TGG 99 3.7297067642211914 20230217174641
- CCTCGGACAGTAATCTGTCT CGG CAGATTACTGTCCGAG TTGGGAAATCTGAGA 5 ATCAGACCCAGGGCAATCGG TGG 99 3.4305777549743652 20230217174641
- CCTCGGACAGTAATCTGTCT CGG CAGATTACTGTCCGAGG AAATCTGAGA 5 ATCAGACCCAGGGCAATCGG TGG 99 3.4123852252960205 20230217174641
- CCTCGGACAGTAATCTGTCT CGG CAGATTACTGTCCGAGG GGAAATCTGAGA 5 ATCAGACCCAGGGCAATCGG TGG 99 3.3924381732940674 20230217174641
- CCTCGGACAGTAATCTGTCT CGG CAGATTACTGTCCGAGG GCTTGGGAAATCTGAGA 5 ATCAGACCCAGGGCAATCGG TGG 99 3.3639163970947266 20230217174641
- CCTCGGACAGTAATCTGTCT CGG CAGATTACTGTCCGA TTGGGAAATCTGAGA 5 ATCAGACCCAGGGCAATCGG TGG 99 3.3119449615478516 20230217174641
- CCTCGGACAGTAATCTGTCT CGG CAGATTACTGTCCGAG CTTGGGAAATCTGAGA 5 ATCAGACCCAGGGCAATCGG TGG 99 3.2524662017822266 20230217174641
- CCTCGGACAGTAATCTGTCT CGG CAGATTACTGTCCGA CTTGGGAAATCTGAGA 5 ATCAGACCCAGGGCAATCGG TGG 99 3.237018585205078 20230217174641
- CCTCGGACAGTAATCTGTCT CGG CAGATTACTGTCCGAGG GAAATCTGAGA 5 ATCAGACCCAGGGCAATCGG TGG 99 3.2354888916015625 20230217174641